Evidence for haploidy in metacyclic forms of Trypanosoma brucei.
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چکیده
منابع مشابه
Evidence for haploidy in metacyclic forms of Trypanosoma brucei.
The parasitic flagellate Trypanosoma brucei undergoes a series of morphologic and metabolic changes during its passage in the digestive organs of its insect vector, a Glossina or tsetse fly. This morphogenesis ends by the differentiation, in the salivary gland of the fly, of the metacyclic form, which will be transmitted in the bloodstream of the mammalian host. On the basis of DNA microfluorom...
متن کاملAnalysis of the variable antigen composition of Trypanosoma brucei brucei metacyclic trypanosomes using monoclonal antibodies.
The metacyclic trypanosomes of a Trypanosoma brucei brucei clone (ILTat 2.1) were analysed with regard to their variable antigen (VAT) composition using monoclonal antibodies. The metacyclic population was antigenically heterogeneous. Despite the heterogeneity, however, the overall VAT composition of the metacyclic population appeared to be limited in number. A similar pattern of reactivity was...
متن کاملCharacterization of the genes specifying two metacyclic variable antigen types in Trypanosoma brucei rhodesiense.
Bloodstream trypanosomes evade the immune system of their mammalian host by sequentially expressing a large number of different variable surface glycoproteins (VSGs). In contrast, metacyclic trypanosomes, the final developmental stage in the tsetse fly, express a much more restricted set of VSGs. These metacyclic VSGs are the first to be exposed to the immune system of the mammalian host after ...
متن کاملThe selenoproteome is dispensable in bloodstream forms of Trypanosoma brucei.
Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei. Both life cycle stages of T. brucei are highly sensitive to auranofin, a compound known to target selenoproteins. However, the same sensitivity is observed in the SepSecS double knock...
متن کاملHighly efficient stable transformation of bloodstream forms of Trypanosoma brucei.
With the completion of the sequence of the megabase hromosomes of Trypanosoma brucei [1], it has become traightforward to design primers and amplify the appropriate anking sequences to knock out genes by homologous recomination [2]. In addition, RNA interference can be used to own-regulate individual genes or gene families [3], and has lso been applied to genome-wide screens [4]. Despite these ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1986
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.83.16.6063